Hybridoma cell line [clone BUSA14] which generates lacZ inducible CD8+ T -cells capable of recognizing both human and mouse gp100(25-33) melanoma antigens presented on dendritic and tumor cell lines.

Relevance: This hybridoma expresses a variety of membrane T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap, and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface.

Production details (How was the model produced): Total splenocytes were isolated from the spleens of Pmel-1 mice and grown in lymphocyte medium. As sensitizing cells, two ml were incubated with 30 µg/ml hgp10025 33 peptide for 2 hours, diluted in 10 ml lymphocyte medium, and added to the flasks. Four days later, Cells were washed once with PBS, separated on Lympholyte-M and fused with the BWZ.36/CD8α cells using polyethylene glycol. For additional details, see the publication.

Host: mice

Tissue: lymphocytes

Conditional: yes, inducible LacZ