A rabbit polyclonal antibody that detects D3 domain of Mucin-2. It targets an epitope within D3

domain from the N-terminal half, which is localized in amino acids 858-1259.

Mucin-2 (MUC2) (UniProt: Q02817; Gene ID: 4583) is a secreted glycoprotein encoded by the MUC2 (SMUC) gene in humans. It forms the primary structural component of the mucus layers lining the intestines, airways, and other mucosal tissues, providing a protective and lubricating barrier against pathogens and particulates.

Mucin-2 is synthesized with a cleavable signal peptide (aa 1–20) and forms disulfide-linked, highly O-glycosylated homotrimers containing four VWFD and two VWFC domains. In the colon, it constitutes both the inner and outer mucus layers—excluding bacteria from the inner layer while undergoing proteolytic cleavage in the outer layer to create a looser matrix that supports bacterial colonization. The abundant O-glycans act as bacterial attachment sites and nutrient sources, shaping the species-specific colonic microbiota.

Reduced Mucin-2 expression, often due to MUC2 promoter methylation, has been associated with early-stage colorectal cancer development.

Immunogen

His-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.

Tested Applications

Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications

Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications