Serum albumins are widely used in medicine, biotechnology, and cultivated meat production. However, they are mostly extracted from animal sources, raising ethical, reproducibility, and scalability concerns. This technology introduces computationally designed human and bovine serum albumin variants with exceptional thermal stability. These variants are expressed at high yields in E. coli, enabling cost-effective, large-scale, animal-free production.
Applications
- Cultivated meat production
- Cell culture reagents
- Molecular biology assays
- Animal-free biotechnology workflows
- Industrial enzyme reactions involving albumin carriers.
- Extending blood-serum half-life of fusion proteins in therapeutics
- Fast and economical
- Large-scale production in E. coli (hundreds of mg/L)
- Stable beyond boiling point – enables purification by heat
- Minimized contamination risks and non-toxic
- Maintains functional ligand-binding capacity
Variants of human and bovine serum albumins (HBA, BSA) were successfully expressed and purified in bacterial systems. Non-toxicity to mammalian cells was validated in HEK293T and hybridoma. Functional performance was validated in both in vitro assays and cell culture models
Stabilized HSA and BSA designs with improved thermal properties, generated through computational modeling for cell culture and biotech applications
Khersonsky O et al., J Mol Biol 2023; 435(17):168191.
