A CRISPR-Cas9–engineered cell line carrying a mutation in the R2-box region of the R2 gene (within the ERE-homologous 5′UTR) exhibits markedly reduced R2 transcript and protein levels. ... Read more

CRISPR-Cas9 is a widely-used tool for genome editing; however, it is very inefficient and often requires the addition of selection markers, forcing the introduction of foreign DNA elements. CRISPR-Cas9 method, while promising, is currently inherently inefficient and requires selec... Read more

CRISPR-Cas9 is a powerful tool for genome editing, widely used for basic research and the development of treatments for genetic diseases. However, its efficiency is currently limited. Gene therapy applications Basic science research Simple implementation – requires o... Read more