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Technology Name
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Scientist
1780
A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and...

A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and tested to determine oil/water content.
This new method allows determining such content rapidly, in non- destructive, specific and quantities analysis of the cores.

Applications


  • Determining the identity and proportions of substances of oil and water content and their distribution in inspected cores

Advantages


  • A non-destructive method which enables to determine the fluid content along the entire length of an intact core or aggregate of cores within their protective sleeves.
  • More comprehensive information and considerable saving of analysis time compared to conventional sampling methods.
    Suitable for all types of rocks including tight-shale rocks.
  • This method enables to measure the weight fraction of oil and water in the core regardless of the core shape, thickness or distribution.
  • The fluid weight fractions in the samples are determined independently, thus the ratio of oil-to-rock weight-ratio is independent of the water content.
  • Due to high penetration of fast neutrons, the method is suitable for screening intact thick rock cores (10-15 cm), for which alternative probes, such as X-rays or slow neutrons suffer limited penetration.

Technology's Essence


In order to map the oil and water content and their distribution, an aggregate of intact cores within their protective sleeves is positioned on a moving conveyor belt and scanned by a broad- energy, fast- neutron beam. The neutrons are detected by a spectroscopic fast neutron imaging detector. The map of neutron-transmission spectra in each pixel provides information of oil/water content and distribution in such cores. 

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  • Prof. Amos Breskin
1571
A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance. There is a...

A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance.

There is a rapidly growing interest in detecting the molecular substrates of social behavior. This interest is driven by the vast implications of such understanding in both research and the pharmaceutical industry, since some prevalent pathological conditions are mainly characterized by a behavioral deficit or abnormality.

It is extremely challenging to quantify social behavior in a reliable manner. Existing methods struggle to find a balance between objectively quantifying behavior on one hand while enabling a natural, stress-free behavioral estimation on the other hand. Currently, researchers work in a strictly controlled and constrained environment that is estranged and stressful to the animals. The outcome is a highly contaminated measurement of natural behavior. This difficultly becomes increasingly complex when more than one animal is involved as often applied in social behavioral studies.

Applications


  • Rigorous characterization of social organizational deficiencies and evaluation of their severity in animal and human models (for example in autism).
  • An optimized system for estimating the efficacy of clinical treatments.

Advantages


  • Long-term tracking of unlimited number of simultaneously studied animals.
  • Machine based, hence objective and automated quantification of behavior.
  • Excellent spatiotemporal resolution in semi natural environment
  • Flexible- the number, size and distribution of the RFID antennas can be adjusted with different enclosure dimensions.
  • Can be applied from Individual behavioral profile or pairs interactions up to collective social organization of groups.
  • Systematic analysis and classification of basic locomotion up to more complex social

Technology's Essence


Researchers at the Weizmann institute developed a method for tightly controlled monitoring of social behavior in a semi-natural environment. They used integrated and synchronized chip reporting and continuous video postage to precisely locate each individual animal. Using this automated monitoring which provides an exceptional temporal resolution they achieved correct identification of numerous basic individual behaviors as well as complex social behaviors. Such complex behavioral profiles set the basis for subsequent analysis which reveals the formation of a social hierarchy.

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  • Dr. Tali Kimchi
1556
Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars. Modern...

Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars.

Modern agriculture faces limited arable land and climate changes. Carbon fixation under these conditions will become a significant growth limiting factor. The proposed solution provides the ability to enhance crop yields using the same expanse of land.

The novel technology presents alternative synthetic carbon fixation pathways that were discovered by harnessing a systems biology approach. These pathways are predicted to harbor a significant kinetic advantage over their natural counter parts, making them promising candidates for synthetic biology implementation.

Applications


  • Synthetic organisms utilizing this revolutionary technology can offer higher carbon fixation rates as compared to natural alternatives allowing:
  • Superior rate of biomass generation, providing cost effective feedstock for the production of biofuels.
  • Enhanced food production via increased crop yields.

Advantages


  • Minimal thermodynamic bottlenecks and superior kinetics over natural counterparts.

Technology's Essence


The productivity of carbon fixation cycles is limited by the slow rate and lack of substrate specificity of the carboxylating enzyme, RuBisCo. In his discovery Dr. Milo addresses the inefficiency of the carbon fixation process through an alternative cycle that is predicted to be two to three times faster than the Calvin–Benson cycle, employing the most effective carboxylating enzyme, phosphoenolpyruvate carboxylase, using the core of the naturally evolved C4 cycle.

A computational strategy was applied, comparing kinetics, energetic and topology of all the possible pathways that can be assembled from all ~4,000 metabolic enzymes known in nature.

The results suggest a promising new family of synthetic carbon fixation pathways.

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  • Prof. Ron Milo
1166
A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

Applications


Monitoring hormone and drug additives in food providing animals for veterinary use and for the food industry.

Technology's Essence


Researchers at the Weizmann Institute of Science have developed a series of mAb against peptide and steroid hormones, isoflavones, and human and veterinary drugs. These antibodies are particularly valuable for monitoring hormone and drug additives in food providing animals. The mAb are available for diagnostics, research, and therapeutics.

The following mAb are available for licensing:

(Clones marked with * are available for diagnostic and therapeutic use only).

Peptide Hormones:
LH: 4F10
bFSH: 1G12*, 1H9, 1H7
FSH: 6H6
bHCG: 1D5
bHCG+: 1C7 3F11
HGH: 1C12*, 1C4*, 5E9, 4E12, 5C3, 1C5, 6G3, 5E6, 2C12

Steroid Hormones:
progesterone-11a-HS 1E11*
progesterone-7a-CET 2H4
Estrone-3-glucuronide 8A3
Testosterone-3-CMO 5A4
Testosterone-3-CMO 5F2*
Estradiol-6-CMO 8D9*

Anti-idiotypic antibodies to anti-steroids:
betatypic anti-anti-testosterone 5A4 8G9
betatypic anti-progesterone 2H4 15F11
betatypic anti-anti-estrone-3-glucuronide 8A3 7C1
alphatypic anti-progesterone 2H4 2E11
betatypic anti-anti-estrone-3-glucuronide 8A3 11C1

Drugs
Digoxin 10F10
RU-486* 8B6*
Buserelin 8B4
Medroxy-progesterone-acetate* 1F5*

Leukotrienes
LTC4* 6E7

Biotin
Biotin-BSA F1

Isoflavones
Daidzein 4E4
Daidzein/daidzin/genistin 2F11
Estrone-3-glucuronide 8A3
Genistein/biochanin A 10D8
Genistein/genistin/daidzin 6E8
Betatypic anti-anti-genistein 10D8

Veterinary drugs
Sulfamethazine (SMZ) 21C7
Betatypic anti-SMZ 12E12
4-chloro-androstenedione 14H2
Virginamycin 486
Spiramycin 110
Betatypic anti-anti-spiramycin 133

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  • Dr. Fortune Kohen
358
Escherichia coli UTL2 Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane. Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane...

Escherichia coli UTL2

Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane.

Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane permeability mutant of Escherichia coli. Proc Natl Acad Sci U S A 93(12):5969-74.

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  • Prof. Eitan Bibi
1378
Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural...

Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural DNA fragments. 

All DNA construction protocols struggle with the cumbersome task of cloning and sequencing synthetic DNA fragments, seeking an error-free one. The problem is worsened for longer synthetic DNA which is more prone to errors. Time spent on error correction, clone selection and sequencing is a major bottleneck that prevents de novo DNA synthesis from becoming a routine procedure in labs. 

This innovative solution significantly decreases the need for labor-intensive time-consuming error correction methods, cloning and sequencing. Furthermore, efficient editing and reassembly of different genes is made possible due to a smart recursive reconstruction process.

 

Applications


  • Design and construction of synthetic biological molecules and organisms.
  • Construction of designer DNA libraries.

 


Advantages


  • Applicable in any lab with standard lab equipment. Faster and more precise than existing methods.
  • Amenable to automation, full synthesis in vitro with a modified smPCR protocol.
  • Very simple to combine synthetic and natural DNA fragments.
  • Does not require additional or external methods or reagents for error correction

 


Technology's Essence


Divide and Conquer (D&C), the quintessential recursive problem-solving technique, is applied in silico to divide the target DNA sequence into overlapping oligonucleotides short enough to be synthesized directly, albeit with errors; error-prone oligonucleotides are recursively combined in vitro, forming error-prone DNA molecules; error-free fragments of these molecules are then identified, extracted and used as new, typically longer and more accurate, inputs to another iteration of the recursive construction procedure; the entire process repeats until an error-free target molecule is formed.

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  • Prof. Ehud Y. Shapiro
237
236-237 - Diastereomer Lytic Peptides for Treatment of Solid Tumors and Metastasis Description: 15-mer (Leu-Lys-Dleu- Leu-Lys-Dlys-Leu-Dleu-Dlys-Lys-Leu-Leu-Dlys-Leu-Leu) and 15-mer Histidin (H-Leu-Lys-D-Leu- Leu-His-D-Lys-Leu-D-Leu-D-Lys-His-Leu-Leu-D-Lys-Leu-Leu-NH2) are membrane-active peptides...

236-237 - Diastereomer Lytic Peptides for Treatment of Solid Tumors and Metastasis

Description: 15-mer (Leu-Lys-Dleu- Leu-Lys-Dlys-Leu-Dleu-Dlys-Lys-Leu-Leu-Dlys-Leu-Leu) and 15-mer Histidin (H-Leu-Lys-D-Leu- Leu-His-D-Lys-Leu-D-Leu-D-Lys-His-Leu-Leu-D-Lys-Leu-Leu-NH2) are membrane-active peptides composed of both D- and L amino acids (diastereomers). These peptides have demonstrated potent anti-cancer and anti metastatic activities in several animal models including models for prostate and lung cancer. They were shown to successfully inhibit tumor growth when injected intratumorally or intraveneously. The 15-mer Histidine form shows reduced systemic toxicity.

References: Papo N, Braunstein A, Eshhar Z, Shai Y. 2004. Suppression of human prostate tumor growth in mice by a cytolytic D-, L-amino Acid Peptide: membrane lysis, increased necrosis, and inhibition of prostate-specific antigen secretion. Cancer Res. 64(16):5779-86.

Makovitzki A1, Fink A, Shai Y. 2009. Suppression of human solid tumor growth in mice by intratumor and systemic inoculation of histidine-rich and pH-dependent host defense-like lytic peptides. Cancer Res. 69(8):3458-63.

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  • Prof. Yechiel Shai
288
hVin monoclonal antibody Description: Monoclonal antibodies raised against human Vinculin purified from human uterus. Specifically stains Vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells. Good reactivity is obtained with human, bovine, chicken, dog, rat mouse, turkey and...

hVin monoclonal antibody

Description: Monoclonal antibodies raised against human Vinculin purified from human uterus. Specifically stains Vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells. Good reactivity is obtained with human, bovine, chicken, dog, rat mouse, turkey and xenopus Vinculin.

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of

several interacting proteins involved in anchoring F-actin to the membrane.

Reference: Geiger B, Volk T, Volberg T, Bendori R. 1987. Molecular interactions in adherens-type contacts. J Cell Sci Suppl.8:251-72.

Applications


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  • Prof. Benjamin Geiger
142
Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.   Biotin: §  116, 142 – Monoclonal antibody to Biotin            Description: Monoclonal...

Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

 

Biotin:

§  116, 142 – Monoclonal antibody to Biotin

           Description: Monoclonal antibodies raised against biotinylated BSA.

           Available clones: F1, F4.

Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). Functions as coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

Reference: Bag?i H1, Kohen F, Kus?uoglu U, Bayer EA, Wilchek M. 1993. Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin. FEBS Lett. 322(1):47-50.

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  • Dr. Fortune Kohen
177
176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115). ERK belongs to the...

176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase

Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115).

ERK belongs to the family of mitogen-activated protein kinases (MAPKs) and is an important component in many intracellular signaling events. ERK is phosphorylated and activated by the upstream kinases, MEK1 and MEK2 on regulatory Threonin (Thr) and tyrosine (Tyr) residues, which are localized in the activation loop of ERK.

Reference: Yao Z, Dolginov Y, Hanoch T, Yung Y, Ridner G, Lando Z, Zharhary D, Seger R. 2000. Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468(1):37-42.

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  • Prof. Rony Seger
263
Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes. Originally raised against an artificial monolayer of lipid mixtures in, and were shown to specifically label the above domains...

Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes.
Originally raised against an artificial monolayer of lipid mixtures in, and were shown to specifically label the above domains in different cell membranes.
Reference:  Scheffer L, Futerman AH, Addadi L. 2007. Antibody labeling of cholesterol/ceramide ordered domains in cell membranes. Chembiochem 8(18):2286-94.

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  • Prof. Lia Addadi
119
Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products. Rat monoclonal antibodies raised against testosterone 3-(0- carboxymethyl)oxime-BSA. Available...

Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.
Rat monoclonal antibodies raised against testosterone 3-(0- carboxymethyl)oxime-BSA. Available clones: 5F2, 5A4.
Testosterone is the principal male sex hormone and an anabolic steroid. In mammals, testosterone is secreted primarily in the testicles of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.
Reference: Kohen F, Lichter S, Eshhar Z, Lindner HR. 1982. Preparation of monoclonal antibodies able to discriminate between testosterone and 5 alpha-dihydrotestosterone. Steroids. 39(4):453-9.

Monoclonal antibodies for peptide and steroid hormones

Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.

Steroid Hormones:

§  119, 169 – Monoclonal antibodies to Testosterone          

      Description: Rat monoclonal antibodies raised against testosterone 3-(0-   carboxymethyl)oxime-BSA. Available clones: 5F2, 5A4.

Testosterone is the principal male sex hormone and an anabolic steroid. In mammals, testosterone is secreted primarily in the testicles of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.

Reference: Kohen F, Lichter S, Eshhar Z, Lindner HR. 1982. Preparation of monoclonal antibodies able to discriminate between testosterone and 5 alpha-dihydrotestosterone. Steroids. 39(4):453-9.


 

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  • Dr. Fortune Kohen
240
240 - Monoclonal antibody directed to ubiquitinated-H2B Description: Monoclonal antibodies, specific to ubiquitinated-H2B in its ubiquitinated form but not in its unmodified state, or to ubiquitin un-conjugated to H2B. Monoubiquitylated-H2B takes part in almost every molecular process associated with...

240 - Monoclonal antibody directed to ubiquitinated-H2B

Description: Monoclonal antibodies, specific to ubiquitinated-H2B in its ubiquitinated form but not in its unmodified state, or to ubiquitin un-conjugated to H2B. Monoubiquitylated-H2B takes part in almost every molecular process associated with chromatin biology Including transcription initiation and elongation ,DNA damage response and repair, DNA replication, nucleosome positioning, RNA processing and export etc. May be used as a detection tool in western blotting, immunoprecipitation and chromatin immunoprecipitation.

Reference: Shema E, Tirosh I, Aylon Y, Huang J, Ye C, Moskovits N, Raver-Shapira N, Minsky N, Pirngruber J, Tarcic G, Hublarova P, Moyal L, Gana-Weisz M, Shiloh Y, Yarden Y, Johnsen SA, Vojtesek B, Berger SL, Oren M. 2008. The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. Genes Dev. 1;22(19):2664-76.

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  • Prof. Moshe Oren
306
Monoclonal antibody to Runx-3 Description: Monoclonal antibody to Runx-3 raised against the Runx3 peptide TPSTPSPRGSLSTTSHF. Available clones: 62, H8. Runx-3 (Runt-related transcription factor 3) is a transcription factor, and a master regulator of lineage specific gene expression in several important...

Monoclonal antibody to Runx-3

Description: Monoclonal antibody to Runx-3 raised against the Runx3 peptide TPSTPSPRGSLSTTSHF. Available clones: 62, H8.

Runx-3 (Runt-related transcription factor 3) is a transcription factor, and a master regulator of lineage specific gene expression in several important developmental pathways. It binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It functions as a tumor suppressor, and is frequently deleted or transcriptionally silenced in cancer.

Reference: Levanon D1, Bernstein Y, Negreanu V, Bone KR, Pozner A, Eilam R, Lotem J, Brenner O, Groner Y. 2011.  Absence of Runx3 expression in normal gastrointestinal epithelium calls into question its tumour suppressor function. EMBO Mol Med. 3(10):593-604.

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  • Prof. Yoram Groner
144
Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry. Leukotrienes:   Drugs: §  144 - Monoclonal antibody to RU-486 Description: Rat monoclonal...

Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

Leukotrienes:

 

Drugs:

§  144 - Monoclonal antibody to RU-486

Description: Rat monoclonal antibodies raised against RU-486.

     Available clone: 8B6, IgG1.

  RU-486 (Mifepristone) is a synthetic steroid compound with both antiprogesterone and antiglucocorticoid properties. Functions as a progesterone receptor antagonist and used as an abortifacient in the first months of pregnancy, and in smaller doses as an emergency contraceptive.

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  • Dr. Fortune Kohen

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