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Category
Technology Name
Briefcase
Scientist
1848
Single-cell RNA sequencing (RNA-Seq) is a powerful tool to identify and characterize the transcriptome of single cells. While providing unprecedented resolution in terms of studying cells, single-cell RNA-Seq is functionally a descriptive tool, unless combined with gene manipulation. CRISPR-Cas9 is a...

Single-cell RNA sequencing (RNA-Seq) is a powerful tool to identify and characterize the transcriptome of single cells. While providing unprecedented resolution in terms of studying cells, single-cell RNA-Seq is functionally a descriptive tool, unless combined with gene manipulation. CRISPR-Cas9 is a genome-editing technology that enables mutating specific and known targets in the genome by using guide RNAs that match the desired target site. CRISPR can therefore be used to generate single gene knock-outs and to create pooled screens that connect genes to functions. However, single-cell RNA-Seq is not scalable, and CRISPR lacks the resolution to elucidate complex phenotypes.

The research team of Prof. Amit developed a new method which combines the two aforementioned techniques – CRISP-Seq. The method uses the ability of CRISPR-Cas9 to induce site-specific mutations and the power of single-cell RNA-Seq to study gene expression in high resolution. Together they enable to examine gene circuits, pathways and functions affected by interference with numerous genes in a single experiment.

Applications


* Enables both manipulation and study of cells

* High resolution of single-cell gene alterations

* High-throughput data on multiple genes from one experiment


Technology's Essence


CRISP-Seq protocol functions by inducing mutations for one or more target genes, on the single cell level using the CRISPR technology. The vectors used for mutation are barcoded for detection and tracking by RNA-Seq, as well as include probes (e.g. fluorescence) for a visible phenotype that can be used for sorting in flow cytometry. Single-cell RNA-Seq completes the process where the effects on the transcriptome of single or multiple mutations can be examined in high-throughput single cell level.

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  • Prof. Ido Amit
1576
Synthetic genes are commonly used in academic and industrial applications, however in many cases these said genes can be toxic when inserted into the ubiquitously used E. coli. This is problematic as time and money is wasted on attempts to generate genes that will not function in E. coli. The problem...

Synthetic genes are commonly used in academic and industrial applications, however in many cases these said genes can be toxic when inserted into the ubiquitously used E. coli. This is problematic as time and money is wasted on attempts to generate genes that will not function in E. coli. The problem is exacerbated as in many cases it is known whether a gene is toxic until it is inserted into the microorganism. Therefore it would be of great value if genes could be pre-screened to determine toxicity or general stability within E. coli.  

The present technology offers a method to save time and money by avoiding problematic clonings. The PanDaTox database can predict the toxicity or “unclonability” in E. coli of over one and a half million genes from nearly four hundred different microorganisms.

Applications


?  Save time and money by avoiding problematic gene syntheses.

?  Design better metabolic pathways for synthetic biology applications by finding genes that can function together.

?  Antibiotic targets by using the database to find novel toxic compounds that can as anti-microbial agents.


Advantages


·         Simple – the PanDaTox database is simple to use with a straightforward user interface.

·         Extensive – the database covers around 1.5 million different genes from close to 400 different microorganisms.

·         Detailed – including exhaustive amounts of information such as taxanomy, protein information, results of previous experiments (when available), and more.


Technology's Essence


The invention relates to PanDaTox a unique system using an algorithm and database to predict whether genes are toxic in E. coli. The system functions by comparing annotated genomes with different runs of whole genome sequencings of different microbial species. By comparing which portions of the genome were successfully cloned previously into E. coli, PanDaTox can now predict the inherent toxicity of different genes or genetic elements.

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  • Prof. Rotem Sorek
1814
Overview Interactions between molecules are the key for many bio-related processes. The ability to characterize these interactions plays a big role in drug development, computer implemented molecular simulations, and research related to biological processes in general. Most of the molecules that...

Overview

Interactions between molecules are the key for many bio-related processes. The ability to characterize these interactions plays a big role in drug development, computer implemented molecular simulations, and research related to biological processes in general. Most of the molecules that participate in biological processes are chiral. Despite the major role of chirality in molecular interactions, especially in various biological and chemical processes, the enantioselectivity of the interaction is not addressed in a proper manner in most of the current calculations. Recently, it was realized that in chiral molecules charge redistribution, which occurs in any biorecognition event, is accompanied by spin polarization, an effect that is not included in the current methods for calculating molecular interactions. Spin polarization is the degree to which the spin, i.e., the intrinsic angular momentum of elementary particles such as electrons, is aligned with a given direction.

 

The Need

Chirality plays an important role in biology. Various techniques are known for simulating and predicting interactions between molecules. However, the current methods often miss the enantioselectivity of biological interactions and do not provide the correct binding energies. This shortcoming suggests that some essential features are not included in our current description of these interactions. There is thus a need for a modification of the current interaction models for better predictions of molecular interactions in various environments.

The Solution

The technique is based on the relation between charge reorganization in a chiral molecule and spin polarization. Thus, charge polarization, occurring in molecular interactions, may both be used to determine the specific chirality of enantiomers of a given molecule and to improve the prediction of interactions between two chiral objects.

Applications


  • Drug design

  • Molecular simulations

  • Chirality determination of a given molecule or a molecular structure


Advantages


  • Better understanding of chiral selectivity in biological systems

  • Enhanced prediction accuracy for various processes and material interactions

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  • Prof. Ron Naaman
1834
A rapid, label-free biosensor for detecting specific conformation of His-tag labeled proteins, accessible for use in both academic- and commercial-R&D. The fluorescent biosensor detects and labels His-tagged proteins in complex environments, such as living cells, while offering high sensitivity,...

A rapid, label-free biosensor for detecting specific conformation of His-tag labeled proteins, accessible for use in both academic- and commercial-R&D. The fluorescent biosensor detects and labels His-tagged proteins in complex environments, such as living cells, while offering high sensitivity, speed, and cost-effectiveness.

Fluorescent biosensing has become a valuable tool in analytical research, while at the same time the poly-histidine tag (His-tag) has become the most commonly used tag for protein labeling.  However, commercial products for labeling and detecting conformational changes in His-labeled proteins suffer from three main limitations: First is their inability to identify specific changes in the protein conformation. Second, even if such changes are detected, labeling of His-tag proteins is usually very laborious. And third, commercial probes tend to be large and consequently can interfere with the proteins function.  Therefore, there is a need for a small, direct, and non-interfering fluorescent probe for detection of conformational changes.

Dr. Margulies and his team have developed a first of its kind fluorescent molecule that provides detection of changes to the surface of His-labeled proteins. Subsequently, labeling proteins only if they are in a specific conformation. The biosensor exhibits high affinity, excellent signal-to-noise ratio and can be easily applied with no fixation or tedious protocols.

Using the technology, many proteins and specific conformations can now be labeled and studied for the first time (proteins that were already successfully labeled using the biosensor: Calmodulin, B-cell lymphoma 2, G-protein).

Applications


·         Fluorescent detection of specific conformations of His-tag labeled proteins.

·         Direct labeling in both live cell imaging and biochemical assays.

·         Tracking protein-protein interactions in vivo.

·         Localizing proteins and measuring their steady-state concentration in vivo.


Advantages


·         Direct, rapid, and easily applicable.

·         Operate in complex biological environments – live cells or biological media.

·         High affinity and superior signal-to-noise ratio.

·         Small in size - unlikely to interfere with the labeled protein function.      


Technology's Essence


The biosensor presented here is comprised from three main molecular-components: (1) the selective binder (binds to a specific tag, e.g. His-tag); (2) the nonselective binder (binds to the protein surface, as long it is in a specific conformation); and (3) a solvatochromic fluorophore.

The selective binder (1) ensures the sensor will only bind to a tagged protein (in this case, His-tagged protein), regardless of its conformational state. Once the protein folds into the specific conformation, the nonselective binder (2) detects and binds to the modified surface configuration (pre-designed to detect the specific conformation). Now that the local environment of the attached-biosensor and its fluorophore (3) has changed, the fluorophore subsequently emits a distinct and measurable signal.

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  • Prof. David Margulies
1658
Rapid, label free assay for glycan identification, accessible for use in medical diagnostics and biomanufacturing quality control. While glycans hold great promise as biomarkers for several diseases including cancer, technologies that enable rapid and sensitive glycan analysis for diagnosis at “point...

Rapid, label free assay for glycan identification, accessible for use in medical diagnostics and biomanufacturing quality control.

While glycans hold great promise as biomarkers for several diseases including cancer, technologies that enable rapid and sensitive glycan analysis for diagnosis at “point of care” settings are not available.

Dr. Margulies and his team from the Weizmann institute of science developed an optical biosensor that is based on combinatorial detection and produces distinct optical “signatures” for even closely related glycan species.

This invention may be implemented into a single device, which will be simple to operate, to identify many types of glycans in high sensitivity for clinical diagnosis and biomanufacturing quality control processes.

Applications


  • Point of care biosensor device for routine detection of glycan biomarkers from clinical samples.

  • Quality control biosensor device for glycans biomanufacturing.


Advantages


  • Label free, rapid, and easy to integrate into a compact self-contained "point of care" system.

  • Highly sensitive due to the combinatorial effect.

  • A single compound identifies many analytes.

  • Ease of miniaturization for future applications.


Technology's Essence


The present invention is based on a multi-sensor array compound which is composed of a non specific receptor (e.g. boronic acid), at least three chromophores and an anchor. The binding event of this compound to an analyte (i.e. carbohydrates, saccharides) is transduced into a measurable optical signature.

The binding of different analytes distinctly affects the emission of each dye, due to direct optical responses of each dye, as well as conformational changes that affect fluorescence resonance energy transfer (FRET) processes among them. Other photochemical processes that further contribute to the discrimination ability of this innovative compound are photo-induced electron transfer (PET) and internal charge transfer (ICT).

The combination of these effects provides a vast number of unique optical signatures. The pattern recognizer evaluates the responses and through predetermined, programmed, or learned patterns, compares the unique pattern or signature of the measurements to stored patterns for known biomarker or chemical species.

Finally, this design is extremely simple to operate and utilizes a single instrumentation, a single excitation wavelength, and a single incubation step, all of which enable straightforward analysis.

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  • Prof. David Margulies
1780
A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and...

A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and tested to determine oil/water content.
This new method allows determining such content rapidly, in non- destructive, specific and quantities analysis of the cores.

Applications


  • Determining the identity and proportions of substances of oil and water content and their distribution in inspected cores

Advantages


  • A non-destructive method which enables to determine the fluid content along the entire length of an intact core or aggregate of cores within their protective sleeves.
  • More comprehensive information and considerable saving of analysis time compared to conventional sampling methods.
    Suitable for all types of rocks including tight-shale rocks.
  • This method enables to measure the weight fraction of oil and water in the core regardless of the core shape, thickness or distribution.
  • The fluid weight fractions in the samples are determined independently, thus the ratio of oil-to-rock weight-ratio is independent of the water content.
  • Due to high penetration of fast neutrons, the method is suitable for screening intact thick rock cores (10-15 cm), for which alternative probes, such as X-rays or slow neutrons suffer limited penetration.

Technology's Essence


In order to map the oil and water content and their distribution, an aggregate of intact cores within their protective sleeves is positioned on a moving conveyor belt and scanned by a broad- energy, fast- neutron beam. The neutrons are detected by a spectroscopic fast neutron imaging detector. The map of neutron-transmission spectra in each pixel provides information of oil/water content and distribution in such cores. 

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  • Prof. Amos Breskin
1556
Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars. Modern...

Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars.

Modern agriculture faces limited arable land and climate changes. Carbon fixation under these conditions will become a significant growth limiting factor. The proposed solution provides the ability to enhance crop yields using the same expanse of land.

The novel technology presents alternative synthetic carbon fixation pathways that were discovered by harnessing a systems biology approach. These pathways are predicted to harbor a significant kinetic advantage over their natural counter parts, making them promising candidates for synthetic biology implementation.

Applications


  • Synthetic organisms utilizing this revolutionary technology can offer higher carbon fixation rates as compared to natural alternatives allowing:
  • Superior rate of biomass generation, providing cost effective feedstock for the production of biofuels.
  • Enhanced food production via increased crop yields.

Advantages


  • Minimal thermodynamic bottlenecks and superior kinetics over natural counterparts.

Technology's Essence


The productivity of carbon fixation cycles is limited by the slow rate and lack of substrate specificity of the carboxylating enzyme, RuBisCo. In his discovery Dr. Milo addresses the inefficiency of the carbon fixation process through an alternative cycle that is predicted to be two to three times faster than the Calvin–Benson cycle, employing the most effective carboxylating enzyme, phosphoenolpyruvate carboxylase, using the core of the naturally evolved C4 cycle.

A computational strategy was applied, comparing kinetics, energetic and topology of all the possible pathways that can be assembled from all ~4,000 metabolic enzymes known in nature.

The results suggest a promising new family of synthetic carbon fixation pathways.

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  • Prof. Ron Milo
1593
The study of social behavior in groups of mice may have crucial implications for understanding the social aspects of different disorders.  To be executed correctly, group studies require the ability to track individual’s behavior within the group structure. The main challenge of current research tools...

The study of social behavior in groups of mice may have crucial implications for understanding the social aspects of different disorders. 
To be executed correctly, group studies require the ability to track individual’s behavior within the group structure. The main challenge of current research tools is to allow individuals identification while maintaining sufficient resolution for accurate tracking.
The present technology provides a system that utilizes fluorescent fur dyes to differentially mark and track individuals within a group. Using a sensitive color camera and a newly designed tracking algorithm, behavior of groups may be recorded and analyzed with high temporal and spatial resolution.   
The technology further offers a method for characterizing the group’s interactions using the maximum entropy model.

 

Applications


 


Advantages


  • High spatial and temporal resolution – enabled by sensitive color video tracking.
  • Enables high detailed analysis of individual behavior within the group.
  • Suitable for community study of groups - limited only by available fur dyes.
  • Compatible with long-term analysis.
  • Simple, cost effective.
  • Minimal suffering and improved animal welfare.

  • Technology's Essence


    The present technology takes advantage of the fact that mice are nocturnal (active at night) animals, to mark their fur with different fluorescent dyes. Under ultraviolet light, the mice can be accurately and automatically tracked, over a number of days. As the mice are allowed to move freely in an interesting arena for exploration containing ramps, nest boxes and barriers (Figure 1), their trajectory and behavior are recorded using a sensitive color camera.
    The system further includes an image processing module which analyses the recorded images, calculates a spatiotemporal model and the nature of social interactions between individuals.
    Combining detailed behavioral and genetic analysis ate the level of individuals, in association with group analysis, may enable the identification of genetic and neuronal correlates of complex social interactions. 

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    • Prof. Alon Chen
    1571
    A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance. There is a...

    A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance.

    There is a rapidly growing interest in detecting the molecular substrates of social behavior. This interest is driven by the vast implications of such understanding in both research and the pharmaceutical industry, since some prevalent pathological conditions are mainly characterized by a behavioral deficit or abnormality.

    It is extremely challenging to quantify social behavior in a reliable manner. Existing methods struggle to find a balance between objectively quantifying behavior on one hand while enabling a natural, stress-free behavioral estimation on the other hand. Currently, researchers work in a strictly controlled and constrained environment that is estranged and stressful to the animals. The outcome is a highly contaminated measurement of natural behavior. This difficultly becomes increasingly complex when more than one animal is involved as often applied in social behavioral studies.

    Applications


    • Rigorous characterization of social organizational deficiencies and evaluation of their severity in animal and human models (for example in autism).
    • An optimized system for estimating the efficacy of clinical treatments.

    Advantages


    • Long-term tracking of unlimited number of simultaneously studied animals.
    • Machine based, hence objective and automated quantification of behavior.
    • Excellent spatiotemporal resolution in semi natural environment
    • Flexible- the number, size and distribution of the RFID antennas can be adjusted with different enclosure dimensions.
    • Can be applied from Individual behavioral profile or pairs interactions up to collective social organization of groups.
    • Systematic analysis and classification of basic locomotion up to more complex social

    Technology's Essence


    Researchers at the Weizmann institute developed a method for tightly controlled monitoring of social behavior in a semi-natural environment. They used integrated and synchronized chip reporting and continuous video postage to precisely locate each individual animal. Using this automated monitoring which provides an exceptional temporal resolution they achieved correct identification of numerous basic individual behaviors as well as complex social behaviors. Such complex behavioral profiles set the basis for subsequent analysis which reveals the formation of a social hierarchy.

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    • Dr. Tali Kimchi
    358
    Escherichia coli UTL2 Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane. Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane...

    Escherichia coli UTL2

    Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane.

    Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane permeability mutant of Escherichia coli. Proc Natl Acad Sci U S A 93(12):5969-74.

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    • Prof. Eitan Bibi
    1166
    A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

    A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

    Applications


    Monitoring hormone and drug additives in food providing animals for veterinary use and for the food industry.

    Technology's Essence


    Researchers at the Weizmann Institute of Science have developed a series of mAb against peptide and steroid hormones, isoflavones, and human and veterinary drugs. These antibodies are particularly valuable for monitoring hormone and drug additives in food providing animals. The mAb are available for diagnostics, research, and therapeutics.

    The following mAb are available for licensing:

    (Clones marked with * are available for diagnostic and therapeutic use only).

    Peptide Hormones:
    LH: 4F10
    bFSH: 1G12*, 1H9, 1H7
    FSH: 6H6
    bHCG: 1D5
    bHCG+: 1C7 3F11
    HGH: 1C12*, 1C4*, 5E9, 4E12, 5C3, 1C5, 6G3, 5E6, 2C12

    Steroid Hormones:
    progesterone-11a-HS 1E11*
    progesterone-7a-CET 2H4
    Estrone-3-glucuronide 8A3
    Testosterone-3-CMO 5A4
    Testosterone-3-CMO 5F2*
    Estradiol-6-CMO 8D9*

    Anti-idiotypic antibodies to anti-steroids:
    betatypic anti-anti-testosterone 5A4 8G9
    betatypic anti-progesterone 2H4 15F11
    betatypic anti-anti-estrone-3-glucuronide 8A3 7C1
    alphatypic anti-progesterone 2H4 2E11
    betatypic anti-anti-estrone-3-glucuronide 8A3 11C1

    Drugs
    Digoxin 10F10
    RU-486* 8B6*
    Buserelin 8B4
    Medroxy-progesterone-acetate* 1F5*

    Leukotrienes
    LTC4* 6E7

    Biotin
    Biotin-BSA F1

    Isoflavones
    Daidzein 4E4
    Daidzein/daidzin/genistin 2F11
    Estrone-3-glucuronide 8A3
    Genistein/biochanin A 10D8
    Genistein/genistin/daidzin 6E8
    Betatypic anti-anti-genistein 10D8

    Veterinary drugs
    Sulfamethazine (SMZ) 21C7
    Betatypic anti-SMZ 12E12
    4-chloro-androstenedione 14H2
    Virginamycin 486
    Spiramycin 110
    Betatypic anti-anti-spiramycin 133

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    • Dr. Fortune Kohen
    147
    Monoclonal antibodies raised against bhCG. Available clones: 1D5 (IgG1), 1E11 (IgG2b).Human chorionic gonadotropin (hCG) is a hormone produced by the syncytiotrophoblast, a component of the fertilized egg, after conception. Monoclonal antibodies for peptide and steroid hormones Due to their high...

    Monoclonal antibodies raised against bhCG. Available clones: 1D5 (IgG1), 1E11 (IgG2b).
    Human chorionic gonadotropin (hCG) is a hormone produced by the syncytiotrophoblast, a component of the fertilized egg, after conception.

    Monoclonal antibodies for peptide and steroid hormones

    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.

    Peptide Hormones:

    §  147-148 - Monoclonal antibody to hCG

    Description: Monoclonal antibodies raised against bhCG. Available clones: 1D5 (IgG1), 1E11 (IgG2b).

    Human chorionic gonadotropin (hCG) is a hormone produced by the syncytiotrophoblast, a component of the fertilized egg, after conception.

     
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    • Dr. Fortune Kohen
    252
    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products. Anti-idiotypic antibody raised against anti-progesterone-7- BSA.KLH conjugate (clone 2H4)....

    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.
    Anti-idiotypic antibody raised against anti-progesterone-7- BSA.KLH conjugate (clone 2H4). Available clones: 15F11 (betatypic), 2E11  (alphatypic).
    Reference: Fort?ne Kohen, Josef de Boever, Geoff Barnard. 1996.  Noncompetitive immunoassay for small molecules. Immunoassay. Pages  405-421.

    Monoclonal antibodies for peptide and steroid hormones

    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.

    Anti-idiotypic antibodies to anti-steroids:

    §  252-253 - Anti-idiotypic antibodies against anti-progesterone   

          Description: Anti-idiotypic antibody raised against anti-progesterone-7-     BSA.KLH conjugate (clone 2H4). Available clones: 15F11 (betatypic), 2E11 (alphatypic).

                Reference: Fort?ne Kohen, Josef de Boever, Geoff Barnard. 1996. Noncompetitive immunoassay for small molecules. Immunoassay. Pages 405-421.

     
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    • Dr. Fortune Kohen
    101
    Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry. Leukotrienes: §  101 – Monoclonal antibody to LTC4 Description: Rat monoclonal antibodies raised...

    Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

    May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

    Leukotrienes:

    §  101 – Monoclonal antibody to LTC4

    Description: Rat monoclonal antibodies raised against Leukotriene C4-KLH.

    Available clone:  6E7 (IgG1).

    Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing

    smooth muscle contractions and increased capillary permeability.

     
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    • Dr. Fortune Kohen
    315
    315 - TBBL (5-thiobutyl butyrolactone) Description: TBBL (5-thiobutyl butyrolactone) is a chromogenic lipo-lactone substrate for Serum paraoxonases (PONs), used for sera tests of PONs activity, based on their lactonase activity.  The primary enzymatic reaction produces an unstable intermediate that...

    315 - TBBL (5-thiobutyl butyrolactone)

    Description: TBBL (5-thiobutyl butyrolactone) is a chromogenic lipo-lactone substrate for Serum paraoxonases (PONs), used for sera tests of PONs activity, based on their lactonase activity.  The primary enzymatic reaction produces an unstable intermediate that rapidly collapses to release a fluorescent/chromogenic moiety.

    Can be used with a variety of biological samples, for example, serum, cells or cell lysates and suitable for high-throughput screens.

    Serum paraoxonase (PON1) is a high-density lipoprotein(HDL)-associated enzyme with antiatherogenic and detoxification properties that hydrolyzes a wide range of substrates and exhibits a wide range of physiologically important activities, including cholesterol efflux, drug metabolism and the detoxification of Ops (organophosphates).

    Reference: Khersonsky O, Tawfik DS. 2006. Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases. Chembiochem. 7(1):49-53.

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    • Prof. Dan S. Tawfik

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