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Technology Name
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Scientist
1730
Production of carbon nanotube based transistors through a process comprised of identification, selection, and placement of pristine carbon nanotubes in conjunction with standard electrical circuitry.Semiconductor devices are vital to everyday life, however conventional semiconducting materials are...

Production of carbon nanotube based transistors through a process comprised of identification, selection, and placement of pristine carbon nanotubes in conjunction with standard electrical circuitry.
Semiconductor devices are vital to everyday life, however conventional semiconducting materials are quickly approaching their limitations. As devices transition from the microscale to the nanoscale, new techniques for their assembly and testing of their properties must be created. Controllable nanofabrication methods are of increasing importance across a wide field of electronics in everything from energy efficient LEDs in flat-screen monitors to transistors for ultra-powerful computers. Our process presents a novel method for producing high quality nanoscale carbon nanotube based transistors. These methods will be of the utmost importance in the forthcoming nano-revolution.

Applications


  • Produce flawless carbon nanotubes
  • Identify, select, and position nanotubes with precision
  • Room temperature operation
  • High sensitivity
  • High resolution

Advantages


  • Single electron transistor (SET) nanoscale imaging
  • Novel nano-electromechanical devices

Technology's Essence


The principle behind this technology is two-fold: 1) Synthesis and selection method of flawless carbon nanotubes, and 2) their combination with nanoscale electric circuitry to form fully controlled composite nanoscale electronic device.
Selection of the carbon nanotube(s) is assisted by a scanning probe microscope (SPM). A composite electronic device is assembled from two separated chips; a nanotube chip where nanotubes are grown over wide trenches, and a standard circuit chip with electrode contacts surrounding the gates to be measured. The nano-assembly is achieved by inserting an SPM cantilever into a trench on the nanotube chip and placing the circuit chip over a suitable nanotube. Once in place, the nanotube is cut locally by passing a strong current between the electrode contacts, and the composite chip is formed.
This composite electronic device can be used to map electronic potentials with high resolution of 100 nm, high sensitivity of 1microV/Hz1/2, at frequencies of 100 MHz and more and all this at room temperature.

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  • Prof. Shahal Ilani
1679
A novel therapy for Triple Negative Breast Cancer (TNBC) using mAbs combinationBreast cancer is the most common cancer in women worldwide. Triple-negative breast cancer (TNBC) representing about 15% of all breast cancer cases, is the deadliest form of all breast cancer subtypes, and tends to affect...

A novel therapy for Triple Negative Breast Cancer (TNBC) using mAbs combination
Breast cancer is the most common cancer in women worldwide. Triple-negative breast cancer (TNBC) representing about 15% of all breast cancer cases, is the deadliest form of all breast cancer subtypes, and tends to affect women at a younger age. Unfortunately TNBC cannot be treated with the common receptor targeted therapies since it does not express these targets, the estrogen, progesterone and Her2/neu receptors. Therefor systemic treatment options are currently limited to cytotoxic chemotherapy. The lack of effective targeted therapies, resistance to chemotherapy, and early metastatic spread have contributed to the poor prognoses and outcomes associated with TNBC.
The current technology offers a novel therapeutic strategy for TNBC. The application of two novel, noncompetitive antibodies against EGFR, achieves a robust degradation EGFR resulting in tumor inhibition.

Applications


  • Novel and unique antibody targeted therapy for TNBC.
  • The novel anti EGFR antibodies can cooperate synergistically with the currently marketed EGFR antibodies.

Advantages


  • A promising therapeutic scenario to treat TNBC.
  • Enhanced EGFR degradation and improved anti-tumor activity, in contrast to clinically approved anti-EGFR mAbs, which display no cooperative effects.
  • Lysosomal EGFR degradation pathway induced by epitope-distinct antibody mixture may potentially lead to improved therapeutic outcome, and reduced resistance.

Technology's Essence


Prof. Yosef Yarden and his team demonstrated that a combination of novel antibodies that target distinct regions on the human EGF receptor resulted in its robust and synergistic down-regulation, leading to pronounced tumor growth inhibition. Furthermore, the combined mAbs induced lysosomal degradation of EGFR, while avoiding the recycling route. Such irreversible mode of EGFR degradation may potentially increase response rate or delay the onset of patient resistance.
Conversely, combining cetuximab and panitumumab, the mAbs routinely used to treat colorectal cancer patients, did not improve receptor degradation because they are both attracted to the same epitope on EGFR.

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  • Prof. Yosef Yarden
1780
A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and...

A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and tested to determine oil/water content.
This new method allows determining such content rapidly, in non- destructive, specific and quantities analysis of the cores.

Applications


  • Determining the identity and proportions of substances of oil and water content and their distribution in inspected cores

Advantages


  • A non-destructive method which enables to determine the fluid content along the entire length of an intact core or aggregate of cores within their protective sleeves.
  • More comprehensive information and considerable saving of analysis time compared to conventional sampling methods.
    Suitable for all types of rocks including tight-shale rocks.
  • This method enables to measure the weight fraction of oil and water in the core regardless of the core shape, thickness or distribution.
  • The fluid weight fractions in the samples are determined independently, thus the ratio of oil-to-rock weight-ratio is independent of the water content.
  • Due to high penetration of fast neutrons, the method is suitable for screening intact thick rock cores (10-15 cm), for which alternative probes, such as X-rays or slow neutrons suffer limited penetration.

Technology's Essence


In order to map the oil and water content and their distribution, an aggregate of intact cores within their protective sleeves is positioned on a moving conveyor belt and scanned by a broad- energy, fast- neutron beam. The neutrons are detected by a spectroscopic fast neutron imaging detector. The map of neutron-transmission spectra in each pixel provides information of oil/water content and distribution in such cores. 

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  • Prof. Amos Breskin
1749
Our novel technology provides an inexpensive, safe and clean solution for loading and unloading of hydrogen on demand with high potential hydrogen storage capacity. Hydrogen storage is currently the key hurdle to its utilization as an alternative green fuel. Being the smallest molecule, hydrogen is...

Our novel technology provides an inexpensive, safe and clean solution for loading and unloading of hydrogen on demand with high potential hydrogen storage capacity.
Hydrogen storage is currently the key hurdle to its utilization as an alternative green fuel. Being the smallest molecule, hydrogen is highly diffusive and buoyant. Currently, hydrogen is stored physically as a gas, requiring high-pressure tanks, or in liquid form at cryogenic temperatures, both methods require high energy input. Proposed chemical storage systems are based on relatively expensive materials, suffer from poor regeneration after hydrogen release and require elevated temperatures and pressures.
The presented technology utilizes inexpensive and abundant organic compounds that generate hydrogen gas during a chemical transformation. Hydrogen release and the regeneration of the original compound are performed in mild conditions using the same catalyst. This system is a promising candidate to be the basis of compact and cost-effective chemical hydrogen storage platforms.

Applications


  • High potential hydrogen storage capacity (6.6 wt%)
  • Inexpensive and readily available hydrogen carriers (aminoalcohols)
  • Relatively mild release and regeneration conditions

  • Advantages


    • Hydrogen-fueled systems, including fuel cells
    • High capacity hydrogen storage systems

    Technology's Essence


    The technology is based on aminoalcohols that are catalytically converted to cyclic dipeptides, while forming hydrogen gas, using a ruthenium pincer catalyst. Peptide hydrogenation, using the same catalyst, regenerates the aminoalcohol. The same method can be applied with diaminoalkanes and alcohols as well.
    The reaction requires a relatively low organic solvent volume, a catalytic amount of base (KOtBu) for the in situ generation of the active catalyst and mild reaction conditions in terms of hydrogen pressure (50 bar) and temperature (~100 oC). Repetitive cycles of the dehydrogenation-hydrogenation reactions can be performed without adding new catalyst, while maintaining high percentages of aminoalcohol conversion.

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    • Prof. David Milstein
    1704
    Neuropathic Gaucher’s (nGD), is a rare but very severe manifestation of the disease, with a varying degree of involvement of the central nervous system, in addition to systemic symptoms. As of today, there is no cure for these severe conditions. The search for such cure is tremendously hindered by the...

    Neuropathic Gaucher’s (nGD), is a rare but very severe manifestation of the disease, with a varying degree of involvement of the central nervous system, in addition to systemic symptoms. As of today, there is no cure for these severe conditions.
    The search for such cure is tremendously hindered by the unmet need for a reliable biochemical biomarker for nGD.
    The present invention identifies the glycoprotein non-metastatic B (GPNMB) as a potential powerful nGD biomarker for use in early diagnosis, determination of disease severity, as well as a straight forward readout in clinical and preclinical experiments.

    Applications


    Diagnosis and drug development for neuropathic GD

    Advantages


    Straight forward diagnostic tool – based on standard biochemical assays
    Relatively simple clinical procedure – samples are collected from CSF and not brain
    High sensitivity – for the diagnosis of disease severity
    Compatible with preclinical experiments

    Technology's Essence


    Prof. Futerman and his team preformed a quantitative global proteomic analysis (using LC-MS/MS) of cerebrospinal fluid (CSF) samples from four patients with Type 3 GD, to identify mis-regulated proteins, compared with healthy subject.
    Glycoprotein non-metastatic B (GPNMB), a protein that was previously associated with several lysosomal storage disorders, exhibited very high levels (a 42-fold increase) in the CSF of type 3 GD patients.  Two peptides were identified from GPNMB, both located in the non-cytosolic domain, suggesting that GPNMB is cleaved and secreted into the CSF from the brain. LC-MS/MS results were validated by ELISA and by western blot analysis in CSF and in human brain samples.
    Several proof of principle experiments were conducted in order to prove the validity of using GPNMB as a biomarker for monitoring disease state and treatments efficacy in neuropathic GD in patients and mouse models:
    GPNMB levels were shown to be correlated with the severity of type 3 Gaucher’s disease patients, as measured by lower IQ score and lower score in Purdue Pegboard test, assessing eye-hand coordination. In addition, using conduritol b epoxide (CBE)-injection based mouse model that simulate different severities and recovery periods, it was shown that GPNMB levels rapidly rise or decline to reliably reflect progress/remission states of the diseases.

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    • Prof. Anthony H. Futerman
    1800
    A new software tool used for the removal of artifacts from transcranial magnetic stimulation (TMS) triggered electroencephalography (EEG) was developed by the group of Prof. Moses. The combined use of TMS with EEG allows for a unique measurement of the brain's global response to localized and abrupt...

    A new software tool used for the removal of artifacts from transcranial magnetic stimulation (TMS) triggered electroencephalography (EEG) was developed by the group of Prof. Moses.

    The combined use of TMS with EEG allows for a unique measurement of the brain's global response to localized and abrupt stimulations. This may allow TMS-EEG to be used as a diagnostic tool for various neurologic and psychiatric conditions.

    However, large electric artifacts are induced in the EEG by the TMS, which are unrelated to brain activity and obscure crucial stages of the brain's response. These artifacts are orders of magnitude larger than the physiological brain activity, and persist from a few to hundreds of milliseconds. However, no generally accepted algorithm is available that can remove the artifacts without unintentionally and significally altering physiological information.

    The software designed according to the model along with a friendly GUI is a powerful tool for the TMS-EEG field. The software has tested and proven to be effective on real datasets measured on psychiatric patients.

    Applications


    • TMS triggered EEG diagnostics

    Advantages


    • Easy to use software with a GUI
    • Exposes the full EEG from the brain

    Technology's Essence


    The new software tool is based on the observation that, contrary to expectation, the decay of the electrode voltage after the TMS pulse is a power law in time rather than an exponential. A model based on two dimensional diffusion of the accumulated charge from the high electric
    fields of the TMS in the skin was built. This model reproduces the artifact precisely, including the many perplexing artifact shapes that are seen on the different electrodes. Artifact removal software based on this model exposes the full EEG from the brain, as validated by continuously reconstructing 50Hz signals that are the same magnitude as the brain signals.

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    • Prof. Elisha Moses
    1751
    Many cancer cells hijack and remodel existing metabolic pathways for their benefit. Specific targeting of these metabolic dependencies offers cancer patients increased efficiency and minimized side effects. Yet, the complexity of these pathways hinders the identification of targets. The present...

    Many cancer cells hijack and remodel existing metabolic pathways for their benefit. Specific targeting of these metabolic dependencies offers cancer patients increased efficiency and minimized side effects. Yet, the complexity of these pathways hinders the identification of targets.
    The present discovery elucidates the pathway by which argininosuccinate synthase (ASS1) down-regulation confer cancer progression. It shows that decreased activity of ASS1 in cancers supports proliferation by linking excess aspartate to pyrimidines synthesis. Importantly, these studies highlight Citrin (a mitochondrial aspartate transporter) inhibition as a potential method to decrease aspartate levels and selectively target this metabolic pathway in ASS1 depleted cancers.

    Applications


    • Targeted Treatment for ASS1 depleted cancers.

    Advantages


    • Targeted therapy, against a well defined pathway, increases the prospects for success.
    • Selective – targeting cancer metabolic dependency minimizes the chances for healthy cells damage that lead to side effects.

    Technology's Essence


    Cancer cells hijack and remodel existing metabolic pathways for their benefit in what is termed the Warburg effect. Researchers from Dr. Ayelet Erez's lab, at the Weizmann institute of Science, have delineated the metabolic benefit(s) conferred by loss of ASS1 to cancers. In agreement with previous experience, they found that ASS1 deficiency has an additional arginine- independent effect that is directly related to its substrate, aspartate.
    By focusing on the relevant physiological and pathological model systems, it was found that ASS1 deficiency-mediated increase in aspartate levels lead to excessive proliferation through pyrimidine synthesis. The link between the two is provided by CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamylase, dihydroorotase complex) and the mTOR signaling pathway.
    Importantly, the present inventors have found that blocking Citrin, the mitochondrial aspartate transporter, rescues cell proliferation by reducing aspartate levels. Citrin may thus serve as a strong candidate for targeted therapy of ASS1 depleted cancers.   
    Supporting this model, retrospective survival analysis of several cancers reveal that cancers with both decreased ASS1 expression and high Citrin levels have a trend for significantly worse prognosis.

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    • Dr. Ayelet Erez
    1585
    Our scientific team has discovered a method to apply the Gabor Transform to signal processing and data compression. Compared to existing methods that are based on Fourier transform, the new method provides for up to 25% savings in content size for video, audio and images, without any loss in quality...

    Our scientific team has discovered a method to apply the Gabor Transform to signal processing and data compression.

    Compared to existing methods that are based on Fourier transform, the new method provides for up to 25% savings in content size for video, audio and images, without any loss in quality.

    By embracing our method, content providers, ISPs and mobile carriers can achieve major savings in data storage and data transfer costs.

    Applications


    The method can be used in virtually all applications involving data storage, communication and signal processing. One of the main commercial application is for lossy data compression for video, audio and images. Those types of content constitute the bulk of today’s Internet traffic, and improved compression will generate substantial savings in storage and data transfer costs.

    The method also applies to the storage, communication and processing of quantum information and may therefore be expected to have applications in quantum calculations, quantum communication and quantum information processing.


    Advantages


    Existing data compression methods are based on numerical implementations of the Fourier transform, known as FFT, DCT and similar.

    Compared to these methods, Gabor transform method demonstrates a very significant advantage in terms of the size of compressed material.  

    The method provides for up to 25% savings in data size, while keeping the same perceived quality of the content.


    Technology's Essence


    We have discovered the definitive solution to the problem of obtaining accuracy and stability in the Gabor transform.  We realized that there must be an exact informational equivalence between the Gabor transform and the discrete Fourier transform (DFT). The latter is known to provide an exact representation of functions that are band-limited with finite support.  Since the DFT implicitly assumes periodic boundary conditions, to obtain this exact equivalence one needs to modify the Gaussians in the Gabor transform to obey periodic boundary conditions. This leads to Gaussian flexibility with Fourier accuracy --- precisely what has been sought since 1946.

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    • Prof. David J. Tannor
    1643
    Improving beta cell isolation and purification techniques is a critical step towards the development of new cell-based therapies, diagnostic applications and diabetes research. Pancreatic Islets are composed of mixed cell populations, among them beta cells, which represent a major focus of interest due...

    Improving beta cell isolation and purification techniques is a critical step towards the development of new cell-based therapies, diagnostic applications and diabetes research. Pancreatic Islets are composed of mixed cell populations, among them beta cells, which represent a major focus of interest due to their participation in the pathology of diabetes. Various techniques have been suggested to accomplish this step, yet efficient and robust isolation of beta cells remains a challenging task.
    The present invention provides an efficient tag-free isolation method for pancreatic cell sub-types, based on separation according to a newly identified collection of surface markers. These markers are tightly correlated with specific functions, such as insulin production, ensuring enrichment of the desired functionality.
    Probing against the newly identified markers in a combinatorial manner allows high degree of purity without compromising the yield, significantly increasing the amount of purified cells. Finally, the method is compatible with both extracts of pancreatic tissues and stem cells derived cultures, the latter set up high expectations in the diabetes therapy field.

    Applications


    A kit for isolation of distinct pancreatic cell subtypes

    Advantages


    • High purity without compromising the yield of isolated cells.
    • Compatible with a variety of heterogeneous sources including cells extracted from pancreatic tissue, committed lineages of stem cells and cultures of differentiated stem cells.                                               

    Technology's Essence


    Using an innovative high throughput screen, linking specific cell surface markers with a particular functionality (e.g. insulin production), a collection of markers not previously identified in connection with pancreatic cells or with diabetes was found to be consistently expressed in human islets.
    Cell isolation according to the selected markers is performed by exposing the heterogeneous source of cells to specific antibodies that recognize these markers, followed by a choice of sorting techniques such as fluorescence activated cell sorting (FACS).
    The innovative concept of this method is the use of marker combinations, iterating the selection. Only cells that express both markers will be sorted out, thus increasing specificity and reducing contaminations. This increased specificity gives rise to a higher degree of purity without compromising the yield, resulting in larger amounts of isolated cells.
    By applying the initial screen in yet another iteration, additional markers can be added to the selection, to refine the isolation procedure. 
    As this method is generally applicable to the purification of mature as well as pluripotent or partially differentiated beta cell progenitors, it holds great potential for the isolation of clinically relevant cells for treatments of diabetes.

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    • Prof. Michael Walker
    • Prof. Michael Walker
    1555
    Albumin binding probe for extending the lifetime of drugs. Most polypeptide drugs, in particular non-glycosylated proteins of molecular mass less than 50 kDa, are short-lived species in vivo having circulatory half lives of 5-20 min. Drug association with endogenous albumin may be suitable for...

    Albumin binding probe for extending the lifetime of drugs. Most polypeptide drugs, in particular non-glycosylated proteins of molecular mass less than 50 kDa, are short-lived species in vivo having circulatory half lives of 5-20 min. Drug association with endogenous albumin may be suitable for designing an approach to protract the action in vivo of, potentially, any short-lived peptide/protein drug. In doing so two principal obstacles must be overcome: (1) following its conjugation, the probe introduced into a peptide or a protein should have sufficient affinity to albumin to manifest prolonged action in vivo, and (2) in case such covalent introduction results in an inactive product, the latter should be capable to undergo slow reactivation at physiological conditions. The present invention relates to engineering prolonged-acting prodrugs employing an albumin-binding probe that undergoes slow hydrolysis at physiological conditions.

    Applications


    • Prolonging half life of short-lined drugs

    Advantages


    • Prolonging the action of the drug without effecting its activity 
    • A desirable pharmacokinetic pattern

    Technology's Essence


    Since albumin is long-lived in vivo, drugs and endogenous substances that tightly associate with it have lower clearance rates than that of the unbound substances, and exhibit prolonged lifetime profiles in vivo. The present invention is based on a concept according to which a long chain fatty acid (LCFA) like albuminbinding compound is covalently linked to a short-lived amino-containing drug to form a non-covalent drug conjugate capable of associating with albumin in vivo, i.e., a long-lived prodrug that gradually releases the pharmacologically active constituent. This approach has been successfully implemented with several drugs (e.g. insulin, exendin and gentamicin).

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    • Prof. Matityahu Fridkin
    • Prof. Yoram Shechter
    1615
    A new process for the production of catalytic metal coated WS2 nanotubes, using cobalt, palladium, nickel, chromium and noble metals.These metal coated nanotubes were shown to have catalytic activity in different organic reactions including degradation of known organic contaminants (Co coated) and...

    A new process for the production of catalytic metal coated WS2 nanotubes, using cobalt, palladium, nickel, chromium and noble metals.
    These metal coated nanotubes were shown to have catalytic activity in different organic reactions including degradation of known organic contaminants (Co coated) and Suzuki and Heck coupling reactions (Pd coated).
    Since catalytic chemical reactions are at the heart of many processes and industries, and efficient catalysis is essential for both economic and environmental reasons, this development of a new catalytic platform bears a potential to influence many diverse markets.

    Applications


    • New and efficient Pd-based catalysts for diverse reactions.
    • New and efficient crude oil HDS catalysts.
    • New and efficient wastewater purification catalysts.
    • Production of activated hybrid WS2 nanotubes with new properties.
    • Tailoring catalytic nanotubes with different band gaps adjusted to different activation and catalysis applications.

    Advantages


    • Formation of highly active catalytic nanotubes
    • Utilization of the nanotubes' very large surface area
    • Recruiting specific nanotube semiconducting characteristics for special catalysis requirements

    Technology's Essence


    The invention involves deposition of metal nanoparticles on prepared WS2 nanotubes (INT-WS2) in a two stage process involving Pd-nanocrystallites assisted activation followed by electroless plating.
    In this process WS2 nanotubes are synthesized according to known procedures. The nanotubes are then covered by metal nanoparticles in a simple and straightforward procedure resulting with highly active nanotubes which can be utilized as catalysts for various chemical reactions.
    This new hybrid technology opens the way to a new family of highly efficient, tunable catalysts; the INTs large surface area, specific band gap design and choice of metal result in an ability to produce unique tailor-made catalysts, applicable to many different industries. 

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    • Prof. Tenne Reshef
    • Prof. Tenne Reshef
    1593
    The study of social behavior in groups of mice may have crucial implications for understanding the social aspects of different disorders.  To be executed correctly, group studies require the ability to track individual’s behavior within the group structure. The main challenge of current research tools...

    The study of social behavior in groups of mice may have crucial implications for understanding the social aspects of different disorders. 
    To be executed correctly, group studies require the ability to track individual’s behavior within the group structure. The main challenge of current research tools is to allow individuals identification while maintaining sufficient resolution for accurate tracking.
    The present technology provides a system that utilizes fluorescent fur dyes to differentially mark and track individuals within a group. Using a sensitive color camera and a newly designed tracking algorithm, behavior of groups may be recorded and analyzed with high temporal and spatial resolution.   
    The technology further offers a method for characterizing the group’s interactions using the maximum entropy model.

     

    Applications


     


    Advantages


  • High spatial and temporal resolution – enabled by sensitive color video tracking.
  • Enables high detailed analysis of individual behavior within the group.
  • Suitable for community study of groups - limited only by available fur dyes.
  • Compatible with long-term analysis.
  • Simple, cost effective.
  • Minimal suffering and improved animal welfare.

  • Technology's Essence


    The present technology takes advantage of the fact that mice are nocturnal (active at night) animals, to mark their fur with different fluorescent dyes. Under ultraviolet light, the mice can be accurately and automatically tracked, over a number of days. As the mice are allowed to move freely in an interesting arena for exploration containing ramps, nest boxes and barriers (Figure 1), their trajectory and behavior are recorded using a sensitive color camera.
    The system further includes an image processing module which analyses the recorded images, calculates a spatiotemporal model and the nature of social interactions between individuals.
    Combining detailed behavioral and genetic analysis ate the level of individuals, in association with group analysis, may enable the identification of genetic and neuronal correlates of complex social interactions. 

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    • Prof. Alon Chen
    1646
    Dedicated and highly efficient EPR analysis of small volume samples in a range of few µl is now made simple with a novel device invented at the Weizmann Institute of Science. This device features a new ejection mechanism and a unique cold trap which enables collection of all time points in a RFQ series...

    Dedicated and highly efficient EPR analysis of small volume samples in a range of few µl is now made simple with a novel device invented at the Weizmann Institute of Science. This device features a new ejection mechanism and a unique cold trap which enables collection of all time points in a RFQ series in one continuous experiment.
    In order to design and develop inhibitors for therapeutic purposes, the reaction mechanisms of enzymes must be understood. For biological applications, a common methodology of addressing this need is combining Rapid Freeze Quench with Electron Paramagnetic Resonance (RFQ)-EPR, which allows the trapping and analysis of short lived intermediates in chemical reactions. However, commercial RFQ-EPR devices are limited for high field EPR applications due to the demand of large sample volumes for each time point.
    Prof. Goldfarb and her team built a new RFQ apparatus based on microfluidic flow and unique ejection and freezing systems, which can be used for collecting small volume samples in capillaries for high field EPR.

    Applications


    This technology, combined with the variety of W-band high resolution EPR technique (ENDOR, DEER and ESEEM) enables better mechanistic studies of enzymatic reactions, with an emphasis on structural transformations during the reaction, in an efficient and accurate way.


    Advantages


    • Collecting all RFQ time points in one continues experiment.
    • Produce small volume samples in the range of a few µl, and handles small capillaries, for high field ERP.
    • An improved dead time of ~5ms, relative to the commercial RFQs with a typical dead-time of 5–10 ms.
    • Ease-of-use and speed.

    Technology's Essence


    The innovative apparatus consists of two main parts: the microfluidic device and the freeze-quench setup. The microfluidic device comprises a mixer, which mixes the two reacting solutions, a flow path where the reaction occurs, and a sprinkler from which the solution is sprayed out of the device. Prof. Goldfarb and her colleagues improved the common mixing device by adding a fast stream of nitrogen gas which mixes with the ejected reaction solution, and sprays the frozen aerosol out in tiny drops at high speed.
    The innovative RFQ device was planned to have a cold solid surface on which the freezing happens rather than the traditional ejection into a cold liquid, in order to minimize the losses of the frozen solution. Moreover the plate rotates at a speed correlated to the flow speed of the solution, thus samples of different reaction times can freeze on a different radius. The frozen samples are then collected into quartz capillaries.

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    • Prof. Daniella Goldfarb
    1564
    A new recyclable size-selective filtration device. Particle size, chemical purity and dispersion of nanoparticles crucially determine their optical, electronic and chemical properties. Size-selective separation technologies are becoming increasingly important for the development of nanoparticles with...

    A new recyclable size-selective filtration device.

    Particle size, chemical purity and dispersion of nanoparticles crucially determine their optical, electronic and chemical properties. Size-selective separation technologies are becoming increasingly important for the development of nanoparticles with well-defined sizes, which have application in the fields of optoelectronic devices, biomedicine, materials, and catalysis.

    Researchers at the Weizmann Institute have fabricated supramolecular ultrafiltration membranes that can be used for filtration and size-selective chromatography of nanoparticles. The membranes are composed of a self-assembled three-dimensional fibrous network that is held together by reversible non-covalent interactions.

    The membranes are robust, easy to fabricate, and recyclable.

    Applications


    • Size-selective separation of semiconductor and metal nanoparticles
    • Uniformity and monodispersity of nanoparticles in solution.
    • Size exclusion chromatography of nanoparticles in the sub-5-nm size regime.

    Advantages


    • Efficient and inexpensive

    • Fast and easy fabrication

    • Recyclable

    • Self-assembled

    • Dual application regime: filtration and/or chromatography


    Technology's Essence


    The recyclable supramolecular membranes are formed from unique perylene derivatives that are large and flat aromatic molecules. These molecules are insoluble in water and form a 3-D network over a solid support, which can be used for the separation of nanoparticles.

    The filters can be subsequently recycled from this mixture using an organic solvent (e.g. dichloromethane), which separates the membrane material from the water-soluble nanoparticles, and reused without loss of performance.

    This material is hence highly attractive for application in the field of nanotechnology.

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    • Prof. Boris Rybtchinski
    1621
    Novel treatment for angiogenesis-related diseases.Angiogenesis — the growth of new blood vessels from pre-existing vasculature — has an essential role in development, reproduction and repair. Pathological angiogenesis is a common theme in a broad range of diseases such as cancer, autoimmune diseases,...

    Novel treatment for angiogenesis-related diseases.Angiogenesis — the growth of new blood vessels from pre-existing vasculature — has an essential role in development, reproduction and repair. Pathological angiogenesis is a common theme in a broad range of diseases such as cancer, autoimmune diseases, age-related macular degeneration and atherosclerosis. The global market for angiogenesis stimulators and inhibitors is forecast to reach ~US $50 billion by the year 2015. Most of the currently marketed angiogenesis regulators, such as Avastin, typically display modest efficacy and therefore further highlight the great need for the development of novel therapeutics. The current technology presents a novel method to treat angiogenesis-related disorders by modulating apolipoprotein B (ApoB).

    Applications


    • ApoB is a potential therapeutic target for the treatment of cancer and other non-neoplastic diseases.
    • ApoB levels may serve as a biomarker for cancer metastasis.

    Advantages


    • The anti-angiogenic effect of LDL administration was demonstrated in vivo, in zebrafish models, as well as in vitro, in relevant human cells lines.
    • Regulation of ApoB levels may be applied to treat a broad range of angiogenesis-dependent diseases.
    • Detection of ApoB levels can be readily achieved by analysis of body fluids such as blood and plasma.

    Technology's Essence


    Using a high-throughput genetic screen for vascular defects in zebrafish, researchers at the Weizmann Institute of Science have identified a genetic mutation that leads to excessive angiogenesis. The mutated gene is responsible for the assembly of ApoB-containing lipoproteins such as LDL, otherwise known as the ‘bad’ cholesterol. The group has found that low levels of LDL promote the formation of new blood vessels by directly interacting with the VEGF pathway. The outlined technology offers methods to modulate the levels of ApoB in order to stimulate, or inhibit angiogenesis, dependent on the therapeutic strategy. For example, inhibition of angiogenesis by increasing ApoB levels may repress tumor growth and attenuate its metastatic potential. In another application of this technology, increased circulating levels of ApoB can serve as a biomarker for the overproduction of blood vessels, thus enabling early diagnosis of pathogenic states in angiogenesis-dependent diseases.

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    • Prof. Karina Yaniv

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